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Effect of Targeting pathway inter‐connectors on gene expression pattern in Lipopolysaccharide (LPS) Induced Human Peripheral Blood Mononuclear Cells (PBMCs)
Author(s) -
Mendis Chanaka
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb188
Subject(s) - peripheral blood mononuclear cell , lipopolysaccharide , signal transduction , gene expression , gene , biology , microbiology and biotechnology , secretion , immunology , in vitro , genetics , biochemistry
Lipopolysaccharide (LPS) is an endotoxin that induces the secretion of pro‐inflammatory cytokines in many cells and has been known to induce septic shock in humans. This project is aimed at developing a rapid diagnostic method to combat the effects of LPS and eventually other toxins by utilizing signal transduction pathways induced by of LPS in Peripheral Blood Mononuclear Cells (PBMC) as a prototype module. We have identified a set of LPS specific genes in human PBMCs through micro array analysis as well as a central signaling pathways consisting some of the LPS specific genes. Previously identified pathway inter‐connectors such as JNK and p38 were then blocked by specific inhibitors and the gene expression pattern of the same set of LPS specific markers were evaluated. The main objective was to investigate the effect of two inhibitors on the time dependent gene expression pattern and then, the impact on the components that are indirectly associated with the inhibited signaling pathways. In addition, the specific inhibitors were evaluated by correlating the observed gene expression pattern though reverse transcription polymerase chain reactions (RT‐PCR) to the protein expression pattern though enzyme‐linked immunosorbent assay (ELISA) Support or Funding Information N/A