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A systemic approach for evaluating biological function of acetyllysine in Danio rerio
Author(s) -
Kim Sun Joo,
Kwon Oh Kwang,
Sung Eun Ji,
Lee Sangkyu
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb160
Subject(s) - acetylation , danio , lysine , zebrafish , kegg , histone , chromatin , biology , proteomics , biochemistry , bromodomain , microbiology and biotechnology , gene , chemistry , gene expression , transcriptome , amino acid
Zebrafish ( Danio rerio ) is a widely used vertebrate model in genetics and cell biology. Although studies in several mammalian species have been performed, the mechanism of lysine acetylation in D. rerio embryos is incompletely understood. Lysine acetylation is an important post‐translational modification. Since the development of mass spectrometry (MS)‐based proteomics technology, important roles of lysine acetylation beyond histones have focused on chromatin remodeling during the cell cycle and regulation of nuclear transport, metabolism, and translation. Here, we investigated the global acetylome in D. rerio embryos by using an MS‐based system. We identified 351 acetylated peptides and 377 non‐redundant acetylation sites on 189 lysine‐acetylated proteins in 5‐day post‐fertilization (hpf) embryos of D. rerio . Among lysine‐acetylated peptides, 40.2% indicated 3 motifs: (ac)KxxxK, (ac)KxxxxK, and Lx(ac)K. Of 190 acetylated proteins, 81 (42.6%) were mainly distributed in the cytoplasm. Gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses showed that lysine acetylation in D. rerio was enriched in metabolic pathways. Additionally, 17 of 30 acetylated ribosomal proteins were evolutionarily conserved between zebrafish and humans. Our results indicate that acetyllysine might have regulatory effects on ribosomal proteins involved in protein biosynthesis. Support or Funding Information This work was supported by a National Research Foundation (NRF) grant funded by the Korean government (MSIP) (NRF‐2012R1A4A1028835 and ‐2014R1A1A2A16053716).