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Antioxidant Capacity of Selected Vegetables, Nuts and Berries and Their Effects on Interferon Gamma Produced By Salmonella Typhi Parasitized Female Albino Rats
Author(s) -
AKANDE IDOWU SULE,
OLA MOJOYINOLA JOANNA,
SALAKO FAITH OLUGBEMILEKE
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb128
Subject(s) - antioxidant , salmonella typhi , food science , phytochemical , traditional medicine , salmonella , biology , medicine , bacteria , biochemistry , genetics , escherichia coli , gene
Almost all synthetic drugs possess adverse side effects in human body. Natural processes by which the immune system can be improved to combat diseases apart from vaccination remain the best options of preventing and terminating diseases. We investigated the antioxidant capacity and effects of selected vegetables, nuts and berries ( Vernonia amygdalina, Telfairia occidentalis, Amaranthus hybridus , Juglans regia , Anacardium occidentale , Elaeis guineensis, Fragaria ananassa and Vitis vinifera ) on antioxidant system and potentials to improving the immune system responses in a typhoid fever model disease in rat. Extracts of selected vegetables, nuts and berries obtained by the use of the sohxlet extractor were orally pre‐administered to adult female albino rats (n=30), of Wistar strain weighing between 120 to 190 g and divided into 6 groups, before inoculation of Salmonella typhi . Widal test was done to ascertain typhoid induction. Blood samples were collected and used for haematological parameters count and determination of oxidative stress biomarkers activities (GSH, CAT, SOD and MDA), liver and kidney function biomarkers activities using automated analyzer and concentration of interferon gamma (INF γ) using Enzyme linked immunosorbent assay (ELISA). Salmonella typhi colony count was done using faecal samples. Phytochemical screening and the antioxidant activities of the extracts were also determined. Pre‐administration of the extracts maintained steady weight of the parasitized albino rats. Extracts of berries and nuts had the highest in vitro antioxidant capacity compared to the controls. Vegetables improved quality of red cell indices. In vivo antioxidant assay indicated a statistically significant value (p≤0.05) for the extracts of nuts and berries in the levels of GSH compared to the controls. There were no statistically significant difference (p≥0.05) in the levels of liver and kidney function biomarkers between the test and the control groups. Nuts and berries reduced S.typhi colonies and recorded lowered production of INF γ compared to other groups. We conclude that the combined consumption of vegetables, berries and nuts improve immune responses and reduction of oxidative stress. Support or Funding Information SELF SPONSORED