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Roles for MED7 and MED21 in Regulating Assembly of the Mediator‐RNA Polymerase II Holoenzyme
Author(s) -
Sato Shiego,
TomomoriSato Chieri,
Saraf Anita,
Florens Laurence,
Washburn Michael,
Tsai KuangLei,
Asturias Francisco J.,
Conaway Ronald C.,
Conaway Joan W.
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.lb107
Subject(s) - mediator , rna polymerase ii , microbiology and biotechnology , transcription factor ii d , rna polymerase ii holoenzyme , transcription (linguistics) , polymerase , biology , rna polymerase , genetics , chemistry , rna , gene , promoter , gene expression , linguistics , philosophy
A key step in regulation of RNA polymerase II (Pol II) transcription is assembly of the Mediator‐Pol II holoenzyme. Here we dissect the mechanism of assembly of the holoenzyme. Our findings identify a subset of Mediator “head” module subunits that constitute a Pol II binding pocket. In addition, we present evidence that Mediator “middle” module subunits MED7 and MED21 play integral roles in promoting Mediator‐Pol II binding. Through structure‐functions studies of the MED7‐MED21 heterodimer, we identify an evolutionarily conserved “hinge” region critical for this process. A working model for how MED7 and MED21 function together to promote assembly of the Mediator‐Pol II holoenzyme will be presented.