Premium
Saporin Inhibits Adonis‐biotin Stimulation of Proliferation of Cells of Caudate Nucleus of Newborn Lambs
Author(s) -
McBride Shaemion,
Ibe Basil Obijiaku,
Rajaee Arezoo,
Koos Brian
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.992.7
Subject(s) - saporin , cell growth , adonis , biology , microbiology and biotechnology , cell , progenitor cell , biotin , chemistry , cytotoxicity , biochemistry , stem cell , in vitro , art , literature , immunotoxin , poetry
The subventricular zone of the caudate nucleus (CN) contains proliferating progenitor stem cells that may play a role in the greater plasticity of the developing brain. CN cells involved in this reorganizational capacity have not been defined. Rodents are used extensively to study developmental disorders, while larger mammals are seldom studied. We hypothesized that the reorganizational ability of the CN involves the proliferation of CN cells. Primary cells of CN of newborn lambs 3–8 days old were used to study the proliferative potential of CN in vitro. We used Adonis‐biotin, a cell permeable Adonis analog and the ribosome‐inactivating protein conjugate Streptavidin‐SAP (Saporin) as a control for cell growth stimulating properties of the Adonis‐biotin. Adonis‐biotin and Saporin modulate cell proliferation by interacting with cell DNA. Cells in culture were treated with different concentrations (nM); 0.1, 1.0, and 10.0 of Adonis‐biotin, Saporin and equimolar mixture of the Adonis‐biotin and Saporin and the culture medium as control for cell proliferation. Treated cells were incubated at 37°C for 4 days. Cell proliferation was determined each day by cell counting. Effect of these treatments on cell proliferation was also studied by DNA synthesis and immunocytochemistry with DAPI staining. Adonis‐biotin stimulation of cell proliferation was both concentration and time dependent. Treatment of cells with Saporin specifically inhibited cell proliferation such that the co‐treatment with Adonis‐biotin did not abrogate the inhibitory effect of Saporin on CN cell proliferation, suggesting that Saporin had profound toxicity to the cell. This observation was supported by DAPI staining where Adonis‐biotin, but not Saporin, treatment stimulated cell proliferation. Taken together this study shows the involvement of CN cells in the plasticity of basal ganglia of the developing brain. Support or Funding Information Grant #20842 from Department of Obstetrics and Gynecology, UCLA.