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Developmental Window Exists in the Hypoxic Ventilatory Response of Juvenile and Adult Male Rats Following Carotid Sinus Nerve Transection
Author(s) -
Coffee Gregory A.,
Getsy Paulina M.,
Lewis Stephen J.
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.984.3
Subject(s) - carotid body , juvenile , glomus cell , hypoxic ventilatory response , hypoxia (environmental) , carotid sinus , control of respiration , medicine , anesthesia , peripheral chemoreceptors , ventilation (architecture) , chemoreceptor , endocrinology , respiratory system , biology , blood pressure , electrophysiology , chemistry , oxygen , receptor , mechanical engineering , genetics , organic chemistry , engineering
The carotid bodies (CB) are peripheral chemoreceptors that respond to hypoxia by stimulating breathing and increasing blood pressure in order to restore oxygen (O 2 ) levels. The carotid body activates chemosensory afferent terminals of the carotid sinus nerve (CSN), which stimulate the brainstem to increase ventilation. Data from our lab suggests a critical developmental window exists in which the hypoxic ventilatory response (HVR) switches from neonatal to adult. This hypoxic ventilatory response (HVR) seen in male adult rats is blunted after bilateral carotid sinus nerve transection (CSNX). Our objective is to determine the HVR of juvenile male Sprague Dawley rats before and after bilateral CSNX. To test this we performed CSNX at postnatal (P) age P21, and exposed the rats to hypoxia (10% O 2 ) using the BUXCO unstrained, awake, whole‐body plethysmography system four days post surgery. Our results suggest the HVR seen in control juvenile rats is blunted compared to adult rats (n= 12 juvenile and n = 9 adult). Also, after CSNX the juvenile rats exhibit a more diminished HVR compared to adult rats (n = 3 juvenile and n = 9 adult). This data suggests that in control male rats, a critical developmental window may exist in which the carotid body becomes fully sensitized to low blood O 2 , thus allowing the greater HVR seen in the adult. In addition, our data shows that a secondary means of responding to hypoxia after CSNX may not have functionally developed in the juvenile rats. Support or Funding Information NIH PO1‐HL1018710