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MAp44: diffusion from blood to cerebrospinal fluid and intrathecal synthesis
Author(s) -
DortaContreras Alberto J,
PadillaDocal Bárbara,
González Ivonne M Iglesias,
MartínezLarrarte Jose P,
CastilloGonzález William,
GonzálezLosada Cristóbal,
GonzálezArgote Javier,
Jensenius Jens Christian
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.970.1
Subject(s) - cerebrospinal fluid , radial immunodiffusion , albumin , intrathecal , complement system , chemistry , csf albumin , immunodiffusion , complement (music) , diffusion , pathology , serum albumin , medicine , immunology , antibody , biochemistry , anesthesia , gene , physics , complementation , phenotype , thermodynamics
MAp44 is a MBL associated protein also related with pattern recognition molecules of the complement system and regulating the lectin pathway of complement activation. Objective To describe the diffusion pattern from blood to cerebrospinal fluid (CSF) and to explore the possibility to be synthesized in CNS. Methods We measured MAp44 in 60 serum and cerebrospinal fluid (CSF) samples from control patients without neuroinflammation by a sandwich ELISA. Albumin was measured in both biological fluids by immunodiffusion technique. Results CSF and serum values follow a normal distribution by Kolmogorov‐Smirnov test. CSF MAp44 is possible to be 13 times more concentrated than the expected value according to their molecular weight. Conclusions The dynamic of the Map44 diffusion in comparison with albumin according to their Q values indicate that this protein can diffuse from blood to CSF but also it is possible to be synthesized in CNS.