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Chronic Lithium Treatment Induces Novel Patterns of Pendrin Localization and Expression in the Renal Inner Medulla
Author(s) -
Himmel Nathaniel J.,
Wang Yirong,
Rodriguez Daniel A.,
Sun Michael A.,
Blount Mitsi A.
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.968.14
Subject(s) - pendrin , renal medulla , medicine , endocrinology , chemistry , distal convoluted tubule , aquaporin 2 , lithium (medication) , intercalated cell , kidney , medullary cavity , reabsorption , biochemistry , transporter , water channel , mechanical engineering , engineering , inlet , gene
Prolonged lithium treatment is associated with various renal side effects, including hyperchloremic acidosis, and is known to induce inner medullary collecting duct (IMCD) remodeling. In animals treated with lithium, the fraction of intercalated cells (ICs), which are responsible for acid‐base homeostasis, increases as compared to renal principal cells (PCs). In order to investigate the intricacies of lithium‐induced IMCD remodeling, male Sprague Dawley rats were fed a 0.3% LiCl‐enriched diet for 1, 2, 3, 6, 9, or 12 weeks. Urine osmolality began to decrease at 1 week, and from 2–12 weeks, animals were severely polyuric. Beginning at 6 weeks, approximately one‐quarter of cells counted in initial IMCD sections expressed vATPase, an IC marker. These cells were localized in portions of the IM where ICs are not normally found. Pendrin, a Cl − / HCO 3 − exchanger, is normally only expressed in two IC subtypes found in the convoluted tubule, the cortical collecting duct, and the connecting tubule. Beginning at 6 weeks of lithium treatment, we observed novel and various pendrin localization and expression patterns in the IMCD, including co‐expression with AQP4, a PC marker. These data collectively suggest that renal IMCD cell plasticity may play an important role in lithium‐induced IMCD remodeling. Support or Funding Information Norman S. Coplon Extramural Grant ‐ Satellite Healthcare