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Radiofrequency Renal Denervation Decreases Inflammation in Kidney Medulla in Spontaneously Hypertensive Rats (SHR)
Author(s) -
Gao Juan,
Xiao Liang,
Polhemus David J,
Smart Frank,
Mernaugh Raymond,
Goodchild Traci T,
Lefer David J,
Harrison David G,
Kapusta Daniel R
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.964.8
Subject(s) - medicine , endocrinology , cytokine , tumor necrosis factor alpha , kidney , angiotensin ii , denervation , monocyte , blood pressure
We have shown that radio frequency ablation (RF‐ABL) of the renal arteries decreases blood pressure and sympathetic activity in hypertensive SHR. Recently, it has been shown that in angiotensin II‐induced hypertensive mice, surgical renal denervation reduces renal inflammatory cell infiltration, fibrosis, albuminuria and nephrinuria independent of blood pressure. The purpose of this study was to determine if RF‐ABL affects regional localization of inflammatory markers in kidneys of spontaneously hypertensive rats (SHR). SHR (n=8 /group) received bilateral RF‐ABL or Sham‐ABL (Biosense Webster Stockert 70 generator and RF‐probe). After 4wks, kidneys were collected, cortex and medulla separated, and processed for either flow cytometric determination of infiltrating tissue numbers of B cells and T cells (n=8/group) or for measurement of tissue cytokine levels (Bio‐Plex; n=10/group) including Interleukin 2 (IL‐2), IL‐5, IL‐6, IL‐7, IL‐10, IL‐17, Interferon gamma (INF‐γ), Granulocyte Monocyte Colony Stimulating Factor (GM‐CSF), Macrophage Inflammatory Protein‐3a (MIP‐3a), and Tumor Necrosis Factor alpha (TNF‐α). RF‐ABL significantly decreased B cells (56% of Sham‐ABL), CD4 + T cells (55% of Sham‐ABL) and CD8 + T cells (61% of Sham‐ABL) in the medulla, but had no effect on the numbers of B and T cells in the cortex. At 4 weeks following RF‐ABL, tissue levels of IL‐2, IL‐6, IL‐7, IL‐10, IL‐17, INF‐γ, MIP‐3a, and TNF‐α were decreased in the medulla as compared to Sham‐ABL. These findings demonstrate that RF‐ABL is associated with a preferential reduction of inflammatory markers in the kidney medulla, which may contribute to blood pressure independent effects of RF‐ABL in hypertensive rats. Support or Funding Information AHA 14POST20450173 to JG; Biosense Webster IIS‐175 to DRK & FS; P30GM106392 to DRK. 1R01 HL092141, 1R01 HL093579, 1U24 HL 094373, and 1P20 HL113452 to DJL.