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The Predominant Role of Kidney in Sex Difference in Ang II‐Induced Hypertension
Author(s) -
Wang Lei,
Chen Rongjun,
Zhang Jie,
Rong Song,
Wang Shaohui,
Wei Jin,
Yip Kaypong,
Liu Ruisheng
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.963.4
Subject(s) - kidney , basal (medicine) , medicine , endocrinology , blood pressure , significant difference , kidney transplantation , kidney transplant , urology , insulin
Hypertension affects over 25% of American adults and is the leading cause of cardiovascular diseases. Men are observed with a greater prevalence for hypertension and cardiovascular diseases than premenopausal age‐matched women. The significant role of kidney in the development of hypertension has been demonstrated both in patients and animal models. However, it is still not clear whether kidneys determine the gender difference in hypertension. We hypothesize that kidney plays a predominant role in the sex difference in Ang II‐induced hypertension. Kidney transplantations were performed between male and female C57BL/6 mice. Male mice received kidney transplants from female donors (F/M, n=5) and female mice received kidney transplants from male donors (M/F, n=5). Allograft kidney transplantations between the same sex were used as controls (M/M, n=3; and F/F, n=3). The remaining native kidney was removed 7 days after transplantation. Radio‐telemetry transmitter was implanted to measure the mean arterial pressure (MAP) 10 days after native kidney removal. After another 10 days recovery, basal MAP was recorded for 5 days, followed by implantation of minipumps subcutaneously for Ang II infusion. No significant difference was found in basal MAP between the M/M and F/F (103 ± 2 mmHg vs 100 ± 3 mmHg) and neither between the F/M and M/F (105 ± 3 mmHg vs 110 ± 2 mmHg). Slow‐presser dose of Ang II (600ng/kg/min) was administrated through implanted minipumps for 14 days. The increase in MAP, as compared to basal MAP, was 31 ± 1.3 mmHg in M/M, 12 ± 2.4 mmHg in F/F, 33 ± 3.3 mmHg in M/F, and 22 ± 2.8 mmHg in F/M. Gender difference of Ang II‐induced hypertension was observed between M/M and F/F. Female kidney in male body (F/M) attenuated the development of hypertension compared to M/M. Male kidney elevated the MAP by similar amount in response to Ang II infusion no matter that it was housed in male ( M/M) or female body (M/F). Our results indicate that kidneys paly a predominant role in the sex difference in Ang II‐induced hypertension. Support or Funding Information This work was supported by Nation Institutes of Health Grants R01DK098582 and R01DK099276