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Involvement of Inhibitor Kappa B Kinase 2 in the Elevated Vascular Tone of Spontaneously Hypertensive Rats
Author(s) -
Choi SooKyoung,
Lim Mihwa,
Kwon Youngin,
Lee YoungHo
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.959.7
Subject(s) - mesenteric arteries , medicine , phosphorylation , vascular smooth muscle , tone (literature) , endocrinology , blood pressure , cardiology , artery , chemistry , smooth muscle , biochemistry , art , literature
Hypertension is one of the leading causes for cardiovascular disease worldwide. A contributing factor to hypertension is elevated vascular tone in small arteries and arterioles. The 20 KDa myosin light chain (MLC 20 ) phosphorylation is determinant of vascular tone. Recently, it has been reported that inhibitor kappa B kinase 2 (IKK2) is involved in the regulation of MLC 20 phosphorylation. We tested whether IKK2 is involved augmented vascular tone and increased blood pressure in spontaneously hypertensive rats (SHRs). Methods and Results The vascular diameter was measured in mesenteric arteries from 16‐week old male SHRs and their control animals, wistar kyoto rats (WKYs). Western blot analysis was performed to determine changes in expression of IKK2, phosphorylation of inhibitor of kappa B alpha (IκBα), and phosphorylation of MLC 20 . We found that IC 50 values of IKK2 inhibitor, SC‐514 were significantly higher in mesenteric arteries from SHRs compared to those from WKYs. Furthermore, expression of IKK2 is significantly increased in mesenteric arteries from SHRs, which is associated with enhanced phosphorylation of MLC 20 . Conclusion Our results show that IKK2 expression and activation are enhanced in the mesenteric arteries of SHRs compared to WKYs. Thus, we suggest that IKK2 contributes to increased vascular tone in SHRs.