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Purinergic Signaling In Rat Mesenteric Arteries In Vivo
Author(s) -
Aalkjaer Christian,
Mazur Aleksandra,
Matchkov Vladimir
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.945.16
Subject(s) - purinergic receptor , prazosin , mesenteric arteries , suramin , vasodilation , medicine , endocrinology , yohimbine , stimulation , chemistry , receptor , artery , antagonist
The role of ATP for vascular function is well documented. In this study we wanted to assess the role of purinergic signaling for rat small mesenteric arteries in vivo. Rats were anesthetized with Ketamin/Xylazin and the intestine exteriorized through a laparotomy. A small mesenteric artery segment was positioned in a 200 μl reservoir and the diameter monitored. The mean diameter of the arteries (μm) was 241±15 (n=12). Application of l‐NAME, indomethacin, TRAM‐34 and apamin resulted in significant constriction (diameter 180±12 (n=12)). Addition of the broad purinergic receptor blocker suramine under these conditions resulted in dilation (diameter 180±19 and 276±19, without and with suramine, respectively (n=6)). During TTX sensitive electrical field stimulation a frequency dependent constriction was seen. At 16 Hz stimulation the response was inhibited by the a1‐receptor blocker prazosin but also by suramin plus the P2X1 inhibitor NF944. The prazosin sensitive constriction to exogenous norepinephrine (NE) was also inhibited by suramin plus NF944. We conclude that rat mesenteric arteries have vasodilator tone from the endothelium and ATP receptor dependent vasoconstrictor tone in vivo and that tone development to both endogenous and exogenous NE is dependent on purinergic receptors.