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The Effect of Adenosine A 1 ‐Receptor Agonists on Regional Haemodynamics in Conscious Freely Moving Rats
Author(s) -
Woolard Jeanette,
Wheal Amanda Jane
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.941.8
Subject(s) - medicine , bolus (digestion) , adenosine , anesthesia , heart rate , saline , hemodynamics , mean arterial pressure , adenosine a1 receptor , adenosine receptor , blood pressure , receptor , agonist
Endogenous adenosine acts as a local mediator in cardiovascular system to protect against the influences of stress, including episodes of ischaemia and reperfusion injury, coronary and ventricular dysfunction, acute myocardial infarction and chronic heart failure1. Here we have compared the effect of a number of A 1 adenosine receptor‐agonists on heart rate (HR), mean arterial pressure (MAP) and vascular conductance in the renal, mesenteric and hindlimb vascular beds in conscious freely‐moving rats. Studies were performed on Sprague‐Dawley rats chronically instrumented with miniature pulsed Doppler flow probes (for assessment of regional haemodynamics) as described previously2,3. Experiments commenced at least 24h after the last surgical procedure. All procedures were carried out with approval of the University of Nottingham Local Ethical Review Committee, under Home Office Project and Personal License Authority. All compounds were prepared in a polyethylene glycol (PEG)‐ based buffer (5% PEG, 2% Tween, 0.9% saline). Agonists were evaluated in the presence or absence of 1,3,dipropyl‐8‐cyclopentylxanthine (DPCPX) (0.1 mg/kg; given as a bolus of 0.1ml flushed with 0.2ml saline) or vehicle. For example, 2‐Chloro‐N 6 ‐cyclopentyladenosine (CCPA) was administered as 3 min infusions of 120, 400 and 1200 ng/kg/min administered over a total of 9 min at an infusion rate of 0.1ml/min one hour following the bolus injection of DPCPX or vehicle. CCPA produced a graded decrease in HR, MAP, renal and mesenteric vascular conductances, but an increase in hindlimb vascular conductance ( Figure). All responses were antagonised by DPCPX ( Figure). A comparisons of the response of a range of A1‐agonists will be presented. Support or Funding Information We thank the British Heart Foundation for financial support.Effect of CCPA on regional cardiovascular haemodynamics.