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EVIDENCES OF ANTIHYPERTENSIVE POTENTIAL OF EXTRACT FROM SOLANUM CAPSICOIDES ALL. IN SPONTANEOUSLY HYPERTENSIVE RATS
Author(s) -
Simões Letícia Oliveira,
Silva Ana Quênia Gomes,
ConceiçãoFilho Ginaldo,
Ribeiro Thácio,
Niero Rivaldo,
Filho Valdir Cechinel,
Silva Darizy Flavia
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.941.3
Subject(s) - captopril , antispasmodic , potency , pharmacology , medicine , chemistry , phytochemical , antihypertensive drug , endocrinology , traditional medicine , blood pressure , biochemistry , in vitro
The aims of this study were to characterize the pharmacological activity of the crude extract of S. capsicoides All. (MeOH‐Sc) and its fractions, on the cardiovascular system in spontaneously hypertensive rats (SHR) and their normotensive controls Wistar Kyoto (WKY), and analyze the phytochemical extract of this plant drug. The HPLC indicated the presence of at least two main compounds previously isolated from this plant, Cilistadiol and Cilistol. Intravenous injections MeOH‐Sc (5, 10, 20 and 40 mg/kg) induced hypotension in both mouse strains in a dose‐dependent manner. The reduction was prominent in SHR compared to WKY (** p<0.01) in doses of 40 mg/kg. This hypotensive effect was associated with a tachycardia was significantly greater (* p< 0.05) in animals WKY compared to SHR. In animals receiving MeOH‐Sc orally for 21 days a significant antihypertensive effect was observed in comparison to control. In addition, treatment with captopril and MeOH‐Sc prevented the development of increased of cardiac mass. Significant increase in urine volume of animals treated with MeOH‐Sc (**p<0.001 and *p<0.05) in the fifth and fifteenth day of treatment was observed. Mesenteric artery rings from animals treated with MeOH‐Sc pre‐contracted with Phe, have suffered vasorelaxation by SNP (10‐13‐10‐4M) concentration‐dependent manner with increased potency of pharmacological significant manner (*** p< 0.001) compared to control. The concentration‐response curve of Phe in mesenteric rings of these animals showed a significant decrease (** p <0.01) the power to Phe compared to control. The cumulative addition of MeOH‐Sc (0,001–1000 μg/ml) in the isolated superior mesenteric artery of SHR and WKY rats, in the presence or absence of functional endothelium intact and rings precontracted with phenylephrine (Phe 1μM), induced a vasorelaxation concentration‐dependent. In vascular rings precontracted with depolarizing solution (KCl 80mM), we observed a significant decrease (*p<0.05 and ** p<0.01) of pharmacological potency induced by MeOH‐Sc in both SHR and WKY. Additionally, MeOH‐Sc reversed contractions induced by CaCl2 in depolarizing medium without nominally Ca2+ in both animal strains. When administered cumulatively in the tonic phase of the contraction induced by S‐(−)‐ Bay K 8644 (200 nM), MeOH‐Sc induced significant changes in the values Emax and CE50 besides demonstrating induce their vasorelaxant effect through inhibition of the influx Ca2+ by ROC and SOC. These results suggest that the MeOH‐Sc has hypotensive effect, vasorelaxant and antihypertensive effect potential, possibly due to an influence of the drug on the plant Ca2+ influx in rat vascular smooth muscle. Support or Funding Information Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)