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Evaluation of the embryotoxic and teratogenic effect of the β‐HPC in ovo
Author(s) -
MendozaRivera Brissa,
ReyesEsparza Jorge,
De la CruzCordero Ricardo,
RodriguezFragoso Lourdes
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.933.9
Subject(s) - in ovo , teratology , embryo , andrology , pregnancy , incubation , physiology , toxicology , medicine , chemistry , biology , fetus , biochemistry , genetics
Exposure of drugs during the pregnancy may have different effects on the development of the embryo, depending on the period of the exhibition. Injuries during the division of the zygote and implementation that can have effects such as malformations, dysfunctions, alterations in growth, stillbirth and altered postnatal functions are called embryotoxicity. On the other hand, irreversible lesions that are compatible with life, but which may result in structural or functional abnormalities in the born products, it is called teratogenicity. The objective of this study was to evaluate whether the β‐HPC produces embryotoxic and teratogenic effects in ovo. Eggs were incubated for 24 h at 37°C and 60% humidity. We included the following treatment groups (n=5): control, negative control, positive control (caffeine 10 mg/mL, for embryotoxicity test) and 60, 120, 240 and 480 μg/mL of β‐HPC. 1 mL of albumin was removed and was replaced by 1 mL of treatment, and sealed with paraffin. The embryos were obtained after 48 hours of incubation with the treatment. They were fixed, dehydrated and were stained for observation. For teratogenicity study, has followed the same procedure, only the embryos developed fully until hatch. Once outside of the hatched chickens were analyzed to verify the presence of abnormalities. Embryotoxicity results showed that β‐HPC different concentrations did not induce structural malformations in embryos. However, the highest dose induced a higher stage compared to the control. In the results of teratogenicity was observed that β‐HPC, did not induce malformations in the development of the chicken. However, it was observed that the development of the chickens was lower, concentration‐dependent. The highest concentration (480 mg/mL) induced a smaller stadium. In conclusion the β‐HPC did not induce effects embryotoxic and teratogenic effects, however, can detain the development of chicken, concentration‐dependent.