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Evaluation of the effect of CdTe quantum dots on cell viability
Author(s) -
RodríguezLopez Anahi,
ReyesEsparza Jorge,
Agarwal Vivechana,
RodriguezFragoso Lourdes
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.933.5
Subject(s) - hela , quantum dot , cadmium telluride photovoltaics , viability assay , biocompatibility , nanotechnology , materials science , mtt assay , chemistry , biophysics , cell , biology , biochemistry , organic chemistry
Evaluate the effect of CdTe quantum dots on cell viability of three cell line. Nanotechnology is the study, design, synthesis, manipulation and application of materials, devices and functional systems through control of matter at the nanoscale (1–100 nm). The quantum dots (QDs) are colloidal crystals semiconductor scales 1–10 nm, producing fluorescence of different colors depending on their size. Due to its several applications in areas such as medicine and the pharmaceutical, there has been a need to understand their interaction with biological systems. Parameters such as size, composition, surface charge, and functionalization can be modified to either enhance biocompatibility or reduce their toxic effects. In this work, the cell viability is evaluated in kidney (Hek293), cervical cancer (HeLa) and liver (HepG2) cells by thiazolyl blue tetrazolium bromide (MTT) technique. Cells were seeded in 96‐well plates in culture media respectively. These cells were treated with CdTe quantum dots of different sizes (3 and 10 nm) (pregunta : do you want to put three different sizes as we prepared in the lab or it is only 1 type from SA? ) at concentrations of 0.1, 1, 10, 100 and 1000 ng/ml for 24 hours. In order to determine the effect of the cell proliferation by MTT technique on HeLa cell line. These cells were treated with CdTe‐COOH QDs (by SIGMA ALDRICH) at the same concentrations as was described above, but also evaluated from one to seven days. The CdTe quantum dots (3 nm) were have no effect on reducing cell viability Hek293Q, HeLa and HepG2. We hypothesized that the cytotoxic effect of CdTe QDs may have a little correlation function its size, as indicated by recent studies which have emphasized on the composition of these QDs and the possibility of toxic effects. Although CdTe QDs of 3 and 10 nm caused no decrease in cell viability Hek293, HeLa and HepG2, except, compared to about 10 nm QDs where HeLa had cytotoxic effect from maximum concentrations of 1000 ng/ml. The quantum dots CdTe‐COOH at concentrations of 1 and 10 ng/ml had no effect on cell proliferation HeLa significantly, but there was an increase in cell proliferación of HeLa significant compared to the control group at concentrations of 0.1 and 100 ng/ml which increased by up to 500% of cell proliferation. Although, in the case of HeLa cells treated to concentration of 1000 ng/ml had no effect on cell proliferation, this result is consistent with that obtained in the cytotoxicity assay.