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Zinc Supplementation and Antioxidant Activity in Adult Women in Rural Southern Ethiopia
Author(s) -
Bogale Alemtsehay,
Stoecker Barbara J.
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.919.6
Subject(s) - oxidative stress , antioxidant , placebo , ferric reducing ability of plasma , superoxide dismutase , malondialdehyde , urine , physiology , medicine , zinc , food science , zoology , biology , chemistry , biochemistry , antioxidant capacity , pathology , alternative medicine , organic chemistry
Accumulating evidence suggests that zinc plays a significant role in antioxidant defense mechanisms and protects cellular components from oxidation. This study evaluated effects of 20 mg zinc supplementation for 23 days on antioxidant activity and oxidative stress biomarkers in blood and urine of women from resource limited households. Adult women (n=48) participated in this randomized double blind, placebo‐controlled trial. Participants were randomly assigned to placebo or zinc groups. Socio‐demographic and dietary diversity data were collected at baseline. Blood and urine samples were collected at baseline and end of the study. Enzyme‐linked immunosorbent assay kits were used to measure several antioxidant activity and oxidative stress biomarkers in plasma, erythrocytes and urine. Mean ± SD age of participants was 33 ± 5 and household size was 7 ± 3. Dietary diversity score was 4.5 ± 1 out of 12 food groups. Mean tropical livestock units per household was 2 ± 1. None of the participants reported consuming meat, fish or egg in the 24 h prior to the survey. Ferric reducing ability of plasma (FRAP), 8‐hydroxydeoxyguanosine (8‐OHdG), malondialdehyde (MDA), superoxide dismutase (SOD), urinary 8‐isoprostane and the other biomarkers measured were not changed significantly in response to zinc supplementation. However, increases representing oxidative damage were observed in 8‐OHdG (p = 0.013) and 8‐isoprostane (p = 0.002) in the placebo group. Although antioxidant activity biomarkers did not differ significantly between baseline and endpoint within the zinc‐supplemented group, they tended to remain higher while some oxidative stress markers tended to remain lower in that group than in the placebo group. Thus, the findings warrant further investigation of these biomarkers Support or Funding Information Supported by Oklahoma State University and W3002 Multistate Project