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Circulating Levels of Small Nucleolar RNAs (snoRNA) Are Affected by Zinc Supplementation in Early Pubertal Females
Author(s) -
Grider Arthur,
Lewis Richard D.,
Laing Emma M.
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.919.4
Subject(s) - small nucleolar rna , zinc , rna , microrna , placebo , chromosome , biology , medicine , long non coding rna , microbiology and biotechnology , endocrinology , chemistry , andrology , gene , genetics , pathology , alternative medicine , organic chemistry
The objective of this research was to determine the effects of zinc supplementation on the levels of small nucleolar RNA (snoRNA) in plasma. The subjects (early pubertal females 9 to 13y) consumed 9 mg elemental zinc (n=110) or placebo (n=102) for 4 wks. Plasma was collected from subjects at baseline and 4 wks. Following analysis by zinc atomic absorption, samples were pooled from 5 subjects exhibiting either the greatest change in plasma zinc (zinc supplemented [Zn]) or the least change in plasma zinc (placebo [P]). Total RNA from these samples ([P] baseline n=2; [P] 4wk n=2; [Zn] baseline n=2; [Zn] 4wk n=2) was isolated, and the small, non‐coding RNA in plasma was analyzed by microRNA microarray. The data (log 2 expression) were separated into unsupplemented (UnS; n=6) and zinc supplemented (ZnS; n=2) groups. For each snoRNA, the UnS group exhibited a coefficient of variation ≤ 30%, and the ZnS group exhibited average expression levels ≥ 1.5‐fold higher or lower than the average of the UnS group. Seven snoRNAs were affected by Zn supplementation: SNORD13 (chromosome 2 – 1.5× decrease, chromosome 5 – 1.6× decrease, chromosome 11 – 1.7× decrease, chromosome 10 – 1.6× increase); HBII‐52 (chromosome 15 – 1.6× decrease); 14q(0) (chromosome 7 – 1.5× increase); SNORD81 (chromosome 7 – 1.6× increase). These results indicate that changes in Zn nutriture affects the circulating levels of this class of small, non‐coding RNAs involved with the post‐transcriptional maturation of ribosomal RNA. Moreover, further research will determine if they are candidates for biomarkers of Zn status. Support or Funding Information Georgia Agriculture Experiment Station (GEO00682) to AG; National Institutes of Health, National Institute of Child Health and Human Development (R03 HD54630) to RDL.