Premium
BRAIN DEVELOPMENT AFTER PRETERM BIRTH IS ENANCED BY INCLUDING PHOSPHATIDYLSERINE IN FORMULA: EVIDENCE FROM PRETERM PIGS
Author(s) -
Buddington Randal K,
Chizhikov Victor V,
Sable Jeffrey J,
Sable Helen J,
Iskusnykh Igor Y,
Steshina E Y,
Holloway Zade R,
Buddington Karyl K,
Lifshitz Yael
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.915.6
Subject(s) - medicine , gestation , brain development , full term , pediatrics , cerebellum , physiology , pregnancy , biology , neuroscience , genetics
Brain development is compromised in 40–50% of preterm infants. This is evident from the decreased cerebellar size from MRI imaging, delays in sensory processing and neural development, and an increased risk of hearing loss after preterm birth. In light of the importance of nutrition support for brain development of preterm infants, we tested if development of the brain of pigs delivered prematurely at a stage of development relevant to 32 week preterm infants (92% term; day 105 of 115 d of gestation would benefit from feeding a formula supplemented with phosphatidylserine (1.9 g/L; InCog TM ; Enzymotec, Israel). Controls included siblings fed formula without InCog TM and newborn vaginally delivered term pigs. Event‐related brain potentials (ERP) were recorded the day of delivery and postnatal days 2, 5, and 10 in response to pairs of 50‐ms tones (500‐ms inter‐tone interval) with randomized inter‐pair intervals (IPI) of 1 or 5 seconds during a 30 minute period with randomized inter‐pair intervals (IPI) of 1 or 5 seconds. ERPs (specifically the negative (N1) and positive (P2) deflection components ~100 ms and 200 ms after a stimulus, respectively) were not detected the day of delivery, were discernable at day 2, and at day 10 (term equivalent) were significantly larger and earlier than at days 2 and 5 emergence. The pigs were euthanized at day 10 and the cerebella were removed, weighed and evaluated by immunohistochemistry. The formula with InCog TM resulted in increased cerebellar weight and numbers of granule cells compared with control formula. These findings suggest InCog TM is a promising ingredient for preterm formula that will improve cerebellar growth and maturation after preterm birth. Support or Funding Information This study was partially funded by Enzymotec