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Intracerebroventricular Oxytocin Effects on mRNA Abundance Differ in Adipose Tissue of Chicks from Low and High Body Weight‐Selected Lines
Author(s) -
Koskinen Anna,
McConn Betty,
Wang Guoqing,
Siegel Paul B,
Cline Mark A,
Gilbert Elizabeth R
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.915.24
Subject(s) - adipose tissue , medicine , endocrinology , biology , oxytocin , chemistry
Neuropeptide oxytocin (OXT), an important anorexigenic factor in the central nervous system, has been shown to have effects on adipose tissue lipid metabolism in mammals. The purpose of this study was to determine the effect of intracerebroventricular (ICV) administration of OXT on subcutaneous adipose tissue mRNA abundance in chicks from lines selected for low (LWS) or high (HWS) juvenile body weight. These lines differ in adiposity. At 5 days post‐hatch, LWS and HWS chicks were fasted for 3 hours, injected ICV with 0 (vehicle), 0.039, 0.312, or 2.5 nmol OXT (n=10 per group).Their food intake was monitored, and subcutaneous adipose tissue was collected at 3 hours post‐injection. Total RNA was isolated, reverse transcribed and real time PCR performed to measure mRNA abundance of the following adipose metabolism‐associated factors: acyl‐CoA dehydrogenase, long chain, 1‐acylglycerol‐3‐phosphate O‐acyltransferase 9, CCAAT/enhancer‐binding protein alpha, CCAAT/enhancer‐binding protein beta, fatty acid binding protein 4 (FABP4), lipoprotein lipase (LPL), monoglyceride lipase (MGLL), OXT, peroxisome proliferator‐activated receptor gamma, and sterol regulatory element‐binding transcription factor 1. The relative quantity values were analyzed by ANOVA in JMP Pro 11 (SAS Inst.). The model included the main effects of genetic line, OXT treatment, and the interaction between them. Significant interactions prompted ANOVAs within each line, and means were separated by Tukey's test. Abundance of FABP4 ( P = 0.02), MGLL ( P = 0.0002), and OXT ( P = 0.001) mRNA was greater in LWS than HWS chicks. There were no main effects of OXT treatment, however there were two‐way interactions of genetic line and OXT treatment on LPL ( P = 0.003), MGLL ( P = 0.02), and OXT ( P = 0.02) mRNA. ANOVAs conducted within each line revealed an effect of treatment on LPL ( P = 0.004), MGLL ( P = 0.02), and OXT ( P = 0.04) in LWS, but not HWS ( P > 0.05 for all three genes). In LWS, LPL mRNA was greater in the fat of chicks treated with 0.312 nmol OXT than in those treated with 0.039 or 2.5 nmol ( P < 0.05), whereas expression of MGLL was greater in chicks treated with 0.312 nmol OXT than in those injected with vehicle or 2.5 nmol OXT ( P < 0.05). Oxytocin mRNA was greater in the fat of LWS injected with 0.312 nmol OXT than in those injected with 2.5 nmol OXT ( P < 0.05). Results show that central administration of oxytocin to young chicks may have effects on adipose tissue physiology and that they are genetic line‐dependent. Support or Funding Information Funding for this work was provided in part, by the Virginia Agricultural Experiment Station and the Hatch Program of the National Institute of Food and Agriculture, U.S. Department of Agriculture