Premium
STUDY OF HEMATOPOIESIS IN PROTEIN RESTRICTION: CHARACTERIZATION OF BONE MARROW STROMAL CELLS
Author(s) -
Santos Ed Wilson Batista,
Dias Carolina,
Silva Graziela Batista,
Oliveira Dalila Cunha,
Beltran Jackeline Soares Oliveira,
Hastreiter Araceli Batista,
ParedesGamero Edgar Julian,
Borelli Primavera Batista
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.915.23
Subject(s) - bone marrow , stromal cell , haematopoiesis , stem cell , erythropoiesis , immunology , immunophenotyping , hematopoietic stem cell , stem cell factor , biology , pathology , chemistry , microbiology and biotechnology , cancer research , medicine , anemia , flow cytometry
Background Protein Restriction (PR) results in pathological changes that are associated with bone marrow (BM) hypoplasia and alterations in the BM microenvironment, with changes of extracellular matrix components, impairing cell cycle, reducing the production of precursors both erythroid and leukocytes, leading to anemia and leukopenia. As bone marrow stroma is important for hematopoiesis we evaluated establishment of bone marrow stroma, as well the proliferation, differentiation and maturation. Material and Methods Bone marrow cells from C57BL/6 mice at 7, 14, 21 and 28 days of culture were used. Characterization of bone marrow populations and the kinetics of stromal establishment were performed by immunophenotyping and stain of cells ( oil red, alizarin red and alkaline phosphatase ). Results Long‐term bone marrow culture (LTBMC) was altering the patern, decreasing the number of hematopoietic (erythroid, megakaryocyte and granulocyte) cells, but maintaining percentage of monocytes/macrophages, and hematopoietic stem cells. On other hand, increase of percentage of adipocytes and osteoblasts were observed. Conclusion PM produces changes in stromal cellularity of LTBMC, which can lead to alterations in the homeostasis and maintenance of the hematopoietic stem cell. Support or Funding Information This work was financially supported by the Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP 2012/24505‐1). E.W.S. was supported by a fellowship from CNPq (160959/2015‐2).