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Lean body mass accretion follows a dose‐response to dietary vitamin D in female weanling rats
Author(s) -
Razaghi Maryam,
djekic.ivankovic Marija,
Agellon Sherry,
Lavery Paula,
Weiler Hope A
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.915.1
Subject(s) - weanling , endocrinology , medicine , lean body mass , vitamin , vitamin d and neurology , analysis of variance , zoology , biology , chemistry , body weight
Lean body mass positively correlates to vitamin D status in human infants. This study tested the effect of vitamin D on growth, lean mass, fat and insulin‐like growth factor‐1(IGF‐1). Weanling rats (4 wk; n=6 female/diet) were randomized to receive AIN‐93G diets modified in vitamin D for 8 wk: control AIN‐93G (1 IU vitamin D 3 /g diet) or experimental diets containing vitamin D 3 at 2 or 4 IU/g diet. Food consumption and body weight were measured weekly. Blood was sampled at wk 0, 4 and 8 for assessment of vitamin D metabolites and IGF‐1. Dual‐energy X‐ray absorptiometry was performed in vivo in wk 0, 4 and 8. Differences among groups were tested using ANOVA, Tukey's post hoc test and p<0.05 is considered as statistically significant. No differences in vitamin D status as measured using LC‐MS/MS were observed at baseline. Serum 25‐hydroxyvitamin D in the 4 IU group was significantly different (p<0.01) from control and 2 IU groups at all time points. C‐3 alpha epimer of 25‐hydroxyvitamin D was higher in both experimental groups compared to control (p<0.01). Lean mass and lean mass accretion were significantly higher (p<0.05) in the 4 IU and 2 IU groups at wk 8 compared to control. Weight, body fat and food intake were not affected by diet. A significant difference in serum IGF‐1 was present over time (<0.001) with smaller declines at wk 8 in the 4 IU group (<0.05). This controlled study confirms that achievement of higher vitamin D status supports a lean body phenotype without altering weight gain during a rapid period of growth. 1serum 25(OH)D (A), change in serum IGF‐1(B) and lean mass accretion(C) over 8 wks of feeding diets with 1, 2 and 4 IU/gDiet in weanling female rats (n=6/group)