Premium
Bioaccessibility and cellular uptake of carotenoids, carotenoid precursors and a‐tocopherol in biofortified golden potatoes ( Solanum tuberosum )
Author(s) -
Chitchumroonchokchai Chureeporn,
Diretto Gianfranco,
Giuliano Giovanni,
Failla Mark L
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.914.6
Subject(s) - phytoene , carotenoid , phytoene synthase , lycopene , solanum tuberosum , food science , solanum , chemistry , carotene , chromoplast , botany , lutein , biology , horticulture , biochemistry , chloroplast , plastid , gene
Potatoes ( Solanum tuberosum) transformed to overexpress bacterial phytoene synthase and phytoene desaturase genes and Erwinia lycopene β‐cyclase gene in a tuber‐specific manner have a “golden” phenotype and contain as high as 50% of the RDA of vitamin A per 250g serving. Boiled tubers from two independent transgenic lines of golden potato (16 and 22 μg total carotenoids/g wet wt) and wild type (2 μg/g total carotenoids) were subjected to simulated oral, gastric and small intestinal phases of digestion to estimate the bioacessibility of carotenoids, phytoene, phytofluene and αTC. Retention of compounds of interest after boiling golden potato exceeded 75% with the exception of phytofluene (40%). Partitioning of total carotenoids, provitamin A carotenoids, all trans‐lutein, phytoene, phytofluene and αTC in the mixed micelle fraction was 17–20%, 16–27%, 26–38%, 20–32%, 15%, and 24%, respectively. Apical uptake of the phytochemicals from micelles generated during simulated digestion of boiled potatoes by monolayers of Caco‐2 cells was 14–21% for carotenoids, 43–45% for phytoene, 23–27% for phytofluene, and 53% for aTC after 4h incubation. Results further support the potential of “golden” potato as a staple food for improving vitamin A status of those at risk for deficiency and the bioaccessibility of carotenoid precursors. Support or Funding Information Ohio Agricultural Research and Development Center