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Variation in ALDH4A1 , Proline Intake and Plasma Proteins in a Population of Young Adults
Author(s) -
Jamnik Joseph,
Kim Mun Heui,
Borchers Christoph,
ElSohemy Ahmed
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.911.5
Subject(s) - proline , single nucleotide polymorphism , biology , population , endocrinology , genetics , medicine , physiology , gene , genotype , amino acid , environmental health
Background The effects of dietary proline content on the lifespan of Caenorhabditis elegans have recently been shown to differ between wild‐type strains and those with a mutation in alh‐6 , a gene involved in proline metabolism. However, this association has not been examined in humans. Objective The objective was to determine whether there is an interaction between dietary proline intake and common genetic variations in the ALDH4A1 gene, the human ortholog of alh‐6 , on proteomic biomarkers in humans. Methods Subjects were Caucasian participants of the Toronto Nutrigenomics and Health Study (n=488), a cross sectional examination of young adults aged 20–29 years. Dietary proline intake was estimated using a 196‐item semi‐quantitative food frequency questionnaire. A panel of 54 plasma proteins that become dys regulated during the progression of many chronic conditions were measured using a multiple reaction monitoring HPLC‐MS/MS assay. Two single nucleotide polymorphisms (SNPs) in the ALDH4A1 gene (rs9117 and rs4912075) were genotyped and the association between these SNPs and the plasma proteome was examined using general linear models (GLMs) adjusted for age, sex, BMI and hormonal contraceptive use among women. GLMs were then used to determine whether stratification by the median value of energy‐adjusted proline intake modified any observed associations. Results There was a significant interaction (p<0.05) between ALDH4A1 variant rs4917025 and proline intake on circulating levels of apolipoprotein I and histidine‐rich glycoprotein, as well as an interaction between rs9117 and proline intake on concentrations of apolipoprotein D, zinc‐α 2 ‐glycoprotein and serum amyloid P‐component (p<0.05). Conclusion These results indicate that genetic variation in ALDH4A1 may interact with dietary proline intake in humans to influence concentrations of various proteomics biomarkers of health. Support or Funding Information Research support from the Advanced Foods and Materials Network.