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Using Untargeted Metabolomics to Elucidate Metabolic Changes in Murine Skin Following Tomato Supplementation
Author(s) -
Cooperstone Jessica L,
Teegarden Matthew D,
Cichon Morgan J,
Tober Kathleen A,
Oberyszyn Tatiana M,
Schwartz Steven J
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.908.1
Subject(s) - carotenoid , phytoene , lycopene , metabolomics , metabolite , hairless , biology , biochemistry , food science , chemistry , bioinformatics
Data from human studies suggests tomato supplementation can modulate erythema and inflammation in skin resulting from ultraviolet light exposure. Carotenoids from tomatoes have received the most attention for this photoprotective effect; however, purified carotenoids appear less effective than whole foods. This suggests other phytochemicals may play a role. Our group has observed that tomato supplementation can decrease tumor number in a murine model of ultraviolet light‐induced keratinocyte carcinoma, suggesting modulation of skin biochemistry by tomato bioactives. The objective of this study was to use untargeted metabolomics to profile and compare the global skin metabolomes of mice fed control and tomato‐containing diets. Male immunocompetent SKH‐1 hairless mice were fed an AIN‐93G diet, an AIN‐93G diet + 10% red tomato powder or a 10% tangerine tomato (a unique tomato variety high in cis ‐lycopene, phytoene and phytofluene that we have shown to be a more bioavailable source of lycopene) powder for 35 weeks. Polar extracts of skin were analyzed to compare differences in both endogenous and exogenous metabolites between the control and tomato fed mice. By employing UHPLC‐QTOF‐MS‐based untargeted metabolomics, we were able to detect over 10,000 compounds in skin, and distinguish metabolite profiles based on diet using multivariate statistics. An understanding of the metabolomic changes in skin with tomato supplementation can elucidate the mechanisms by which this fruit may be exerting protective effects. Support or Funding Information NCI R21CA158625