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Effects of melatonin on lipid metabolism and circulating irisin in diet‐induced obese Sprague‐Dawley rats
Author(s) -
Chiang PeiChin,
Lo ChungCheng,
Lin ChihYi,
Chien Yiwen
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.907.9
Subject(s) - melatonin , medicine , endocrinology , adipose tissue , white adipose tissue , brown adipose tissue , lipid metabolism , chemistry , biology
Globesity refers to the worldwide increase of excess weight and obesity according to World Health Organization. Previous studies indicated melatonin, master clock controlling circadian rhythm, may halt body weight gain by increasing recruitment of brown adipose tissues. In light of the pressing need to check the progression of obesity, our study was aimed at investigating effects of oral melatonin administration on body fat accumulation and lipid parameters. Especially, we analyzed irisin, a novel myokine associated with browning effect of white adipose tissues, as the biomarker of brite/beige adipocytes. Forty 8‐wk‐old male Sprague‐Dawley rats were divided into 5 groups: vehicle control (VC), positive control (PC), MEL10 (10 mg melatonin/kg BW), MEL20 (20 mg melatonin/kg BW) and MEL50 (50 mg melatonin/kg BW). Vehicle control group was fed control diet, and the other groups were fed high fat and high calorie diet for 6 weeks before melatonin treatment to induce obesity. Melatonin was dissolved in ethanol and diluted in drinking water with aluminum foil covering water bottle. Rats were treated for 8 weeks and sacrificed. We found that there is no deference in food and water intake, and melatonin administration can halt body weight gain, decrease accumulation and distribution of white adipose tissues, increase brown adipose tissue and brite/beige adipocytes, and lipid parameters were improved. Serum irisin concentration was significantly increased in melatonin‐treated groups.