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Association between obesity and amino acids: a metabolomics approach
Author(s) -
Marchioni Dirce,
Carioca Augusto Antonio,
Miranda Andreia Alexandra,
Carvalho Aline Martins,
Steluti Josiane,
Cotrim Ismael Dale,
Silva Alexsandro Macedo,
Fisberg Regina Mara
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.907.4
Subject(s) - glutamine , amino acid , waist , isoleucine , valine , national health and nutrition examination survey , medicine , body mass index , obesity , chemistry , endocrinology , leucine , population , biology , biochemistry , environmental health
Obesity has reached epidemic proportions in many countries around the world and is strongly linked to a number of chronic diseases. Some amino acids, such as three branched amino acids and glutamine, are associated with metabolic disorders. The interaction between obesity and amino acids is complex and not yet fully elucidated. The metabolomics can be a useful tool for solving some of these metabolic disorders. The aim was to investigate the association between amino acids and obesity. Methods Data came from a population based cross‐sectional survey in Sao Paulo among 169 adults. A trained research nurse measured body weight, height and waist circumference (WC) using a standardized protocol. BMI was calculated by dividing weight (kilograms) by the square of height (meters). Obesity was determined based on BMI ≥ 30 kg/m 2 . The branch chain amino acids (BCAA) are the sum of the valine, leucine, and isoleucine. The glutaminolysis ratio (Gluta Ratio) is (alanina+ aspartate + glutamate)/glutamine. Quantification of the metabolites was performed by mass spectrometry of the type targeted. Multiple linear regression models were performed to verify the association between amino acids (BCAA and Gluta ratio) and BMI or WC, adjusted for age, sex, energy intake, race, smoking, physical activity. Results The sample was comprised of 52% men, 60% adults and mostly of white race (57%). The BMI was associated with BCAA (β=8.57; p<0.001) and Gluta Ratio (β=0.02; p<0.001). The WC was associated with BCAA (β=3.92; p<0.001) and Gluta Ratio (β=0.01; p<0.001). The concentration of BCAA (β = 82.67, p <0.001) and Gluta ratio (β = 0.211, p <0.001) were higher in obese subjects compared with non‐obese. Conclusion Obesity was associated with three branched amino acids and glutamine metabolism. Support or Funding Information CNPq grant 473778/2010‐6 and FAPESP grant 2011/19788‐1