Premium
Alcohol Interrupts Adipose Tissue Maturation and Attenuates Adiponectin Expression in 3T3‐F442 Pre‐Adipocytes
Author(s) -
Shafiei Mahnoush S,
Juma Shanil,
Prasad Chandan,
Imrhan Victorine,
Vijayagopal Parakat
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.904.26
Subject(s) - adiponectin , adipogenesis , adipose tissue , adipokine , endocrinology , medicine , proinflammatory cytokine , 3t3 l1 , inflammation , lipid droplet , chemistry , tumor necrosis factor alpha , biology , insulin resistance , insulin
Alcohol (ethanol) is a widely used substance and excessive consumption has serious adverse effects. Alcohol disrupts endocrine activity of adipose tissue through synthesis and secretion of a wide variety of peptides and cytokines called adipokines. Adiponectin plays important roles in lipid and carbohydrate metabolism and provides a crucial link between alcohol‐induced inflammation and metabolic complications. We hypothesized that alcohol disrupted adipogenesis resulting in decreases in adiponectin expression and up regulation of pro‐inflammatory cytokines. We differentiated 3T3‐F442A preadipocytes in the presence of different does of ethanol and measured adipogenesis and the expression of NF‐kB, adiponectin and several pro‐ and anti‐inflammatory cytokines. Our result shows that alcohol (i) inhibited adipogenesis in a dose‐dependent manner by suppressing intracellular triglyceride accumulation, (ii) significantly reduced adiponectin expression, (iii) increased expression of the pro‐inflammatory cytokines (IL‐6, IL‐12, TNF‐α) and transcription factor NF‐kB, and (iv) decreased expression of the anti‐inflammatory cytokines (IL‐4, IL‐10). Collectively, our findings demonstrate that alcohol exerts a major influence in adipose tissue maturation and inflammatory status. Support or Funding Information Partial support from the Human Nutrition Research Fund at Texas Woman's University