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In‐vitro Comparative Absorption of Creatine Compounds
Author(s) -
Morgan Shane,
Ericson Clayton,
Hartle Jennifer W
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.898.8
Subject(s) - creatine , absorption (acoustics) , chemistry , chromatography , medicine , biochemistry , materials science , composite material
The purpose of this study was to compare the absorption of two creatine sources in a Caco‐2 in‐vitro model designed to mimic the intestinal absorption of creatine when administered as a dietary supplement. A new proprietary source of creatine, Creatine UltraPower™ (CUP), creatine monohydrate (CRE) and a negative control were evaluated for relative absorption of creatine. Caco‐2 cells (ATCC HTB‐37) were used to seed transwell plates. After seeding, cells were allowed to differentiate for 21 days prior to absorption experiments. Membrane integrity in the transwell was verified by trans‐epithelial electrical resistance measurements just prior to running the assay. Cells were rinsed with warm PBS and creatine test solutions were then applied to apical wells. Concentration of creatine in each of the test solutions was 0.42% (w/v) which delivered 349 μmol of creatine to the apical side of the cell. The cells were allowed to incubate for 2 hours. After incubation, the basal fluids were collected and analyzed for creatine concentration by HPLC using an isocratic method on a C18 column, with detection at 201 nm. CUP showed the highest creatine concentration (0.562±0.00686 mg/mL), which was significantly higher than CRE (0.294±0.00463 mg/mL) (p<0.05). Absorption of both creatine forms were both significantly higher than the negative control. These findings suggest that CUP is absorbed better than CRE in a cell culture model and is transferred to the basal side of the cell layer, which suggests that there is more creatine available for biological functions. Support or Funding Information Albion