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Understanding the Phylogenetic Relationship of Bacterial Arginine Kinases
Author(s) -
Stock Katie,
Aryal Manish,
Fraga Dean
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.856.3
Subject(s) - transferase , kinase , phylogenetic tree , biology , enzyme , arginine , biochemistry , arginine kinase , phylogenetics , bacterial genome size , horizontal gene transfer , genetics , genome , gene , amino acid
Arginine kinases (AKs) are transferase enzymes that are part of the phosphagen kinase family. These enzymes catalyze the transfer of phosphate from ATP to L‐arginine and are important in the energy buffering mechanisms in invertebrates and unicellular organisms. Previous studies have attempted to understand the relationship of the bacterial AKs to other phosphagen ianses. Atleast eight bacterial AKs have been discovered through examining bacterial genome sequences available on‐line. Using phylogenetic analyses it was found previously that the bacterial AKs can be separated into two clusters, cluster A and cluster B. This work will describe a more complete characterization of the bacterial AKs, both biochemically and phylogenetically to better understand their relationships to each other and to other PKs. Our initial results support the observation that there are two ‘forms’ of the bacterial AKs, a kinetically slow form and a kinetically faster form. Support or Funding Information College of Wooster