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Patterns of 16S rRNA Microbial Regions in Aggressor‐Exposed Mice with Features of Post‐Traumatic Stress Disorder
Author(s) -
Gautam Aarti,
Hoke Allison,
Kumar Raina,
Chakraborty Nabarun,
Muhie Seid,
Hammamieh Rasha,
Jett Marti
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.852.2
Subject(s) - microbiome , firmicutes , biology , 16s ribosomal rna , verrucomicrobia , ribosomal rna , metagenomics , genetics , microbiology and biotechnology , gene
We evaluated repeated exposures of mice to a trained aggressor mouse as a model for aspects of post‐traumatic stress disorder (PTSD), which was adapted from “social stress” models of traumatic stress, and used a “cage‐within‐cage resident‐intruder” protocol where subject C57BL/6J mice were exposed to aggressor mice for 10 days. With the increased awareness of the significance of the symbiotic influence of microbiota on the nervous system and behavior, this longitudinal study focused on the effect of social stress on the microbiome. Fecal samples were collected daily during the 10 day exposure time‐course from control and aggressor‐exposed (Agg‐E) mice. DNA was isolated; followed by amplification of variable V3 and V4 region of the 16S ribosomal RNA (rRNA) amplicon using the Illumina 16S Metagenomics Library Preparation Protocol. The samples were barcoded with Nextera index primers and pooled prior to sequencing on the Illumina MiSeq platform. Our results indicate on day 4 in Agg‐E mice, the phylum Bacteriodetes shows lower relative abundance, whereas phyla Firmicutes and Verrucomicrobia exhibit higher abundance. The predicted functional enrichment of abundant bacterial composition corresponds with the PPAR and Adipocytokine signaling pathways being highly activated in Agg‐E mice in comparison to control mice. DISCLAIMERS: Research was conducted in compliance with the Animal Welfare Act and all other Federal Requirements. The views expressed are those of the authors and do not constitute endorsement by the U.S. Army. Support or Funding Information Research supported by CRADA # W81XWH‐11‐0076.