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Invivo efficacy and durability of anti‐infective foley catheters functionalized with silver nano particle and antibiotics
Author(s) -
Srinivasakumar KP,
Mala R,
Annie Aglin A,
Kiruthika S,
VazagaPriya C
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.852.1
Subject(s) - antibiotics , catheter , antimicrobial , in vivo , medicine , pharmacology , chemistry , microbiology and biotechnology , surgery , biology
Urinary catheters are an inevitable medical device in health care unit. Complications of urinary catheterization are dreadful. To circumvent the drawbacks associated with existing catheters, the present investigation is aimed to impregnate urinary catheter with a synergistic combination of silver nanoparticle and antibiotics. The functionalized catheter was evaluated by invitro and invivo methods for its anti‐infectious property. Silver nanoparticle was synthesized using Spirulina platensis and it was characterized by SEM and FTIR. Susceptibility of uropathogenic E.coli to antibiotics and silver nano particle was evaluated by agar well diffusion method. MIC and MBC of silver nano particles and antibiotics against the uropathogen was determined by serial twofold dilution method. Invitro antimicrobial property of functionalized catheter was assessed by serial plate transfer . Invitro antiadhesive property of the functionalized catheter was evaluated by challenging catheter with multidrug resistant uropathogenic E.coli (10 5 cfu/ml). Invivio efficacy of the functionalized catheter was assessed in mice according to the clearance of Institutional Animal Ethical Committee. After catheterization in mice, urine sample was collected regularly for a period of 15 days and it was assessed for the presence of pathogens. Liver function test and kidney function tests of mice were analyzed to understand the host toxicity. The stability of the catheter was evaluated for a period of 18 months post primary studies to validate the stability. The uropathogenic E.coli was identified to be multidrug resistant.It was susceptible to amikacin and nitrofurantoin. Size of the nanoparticle was 42–75nm. UV‐Vis spectrum exhibited an absorbance maximum at 408nm. The MIC value of nitrofurantoin and amikacin were 2 mg/ml and 150×10 −3 mg/ml respectively. Amikacin (40μg/ml) and nitrofurantoin (10μg/ml) showed synergistic effect with 1.176μM of SNP. Invitro anti‐infectious property was evaluated in unfunctionalized catheter, catheter impregnated with amikacin, nitrofurantoin, silver nanoparticles and synergistic combination of antibiotics and silver nanoparticle. All catheters were colonized by the uropathogen except in the synergistic combination. It retained its property till 14 days. In invivo study, the catheter colonization was nil in mice catheterized with the synergistic combination till 15 days. Liver function test and kidney function tests in mice were normal. After 18 months of aseptic preservation, the efficacy was evaluated by invitro methods. It showed an effective zero colonization. Hence, the present study has revealed that impregnation of urinary catheter with a synergistic combination of silver nano particle and antibiotics is an efficient and promising method for preventing Catheter Associated Urinary Tract infection.