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Effects of Paternal High Fat Diet on Plasma Leptin, Adiponectin, and Insulin in Offspring of C57BL/6 Mice
Author(s) -
Zontini Alexis,
Slyvka Yuriy,
Zhang Yizhu,
Nowak Felicia V
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.851.16
Subject(s) - leptin , adiponectin , offspring , medicine , endocrinology , obesity , insulin , sibling , biology , insulin resistance , pregnancy , psychology , developmental psychology , genetics
Parental obesity is a predictor of childhood excess weight. Having a father, mother or sibling with a BMI of 40 increases the risk of adult obesity 5 fold compared to individuals who have normal weight first degree relatives. The rise in obesity in the U.S. has played a major role in the increasing number of instances of cardiovascular disease, diabetes, and even some types of cancer. This study aims to determine phenotypic effects on leptin, adiponectin, glucose tolerance (GTT), and HOMA in offspring of non‐obese (Group 1) and high‐fat diet‐induced obese (Group 2) fathers in C57BL/6 mice. Male mice were fed a low fat diet (LFD, 10% fat) or a high fat diet (HFD, 45% fat) for 12 weeks then mated to females fed LFD. Pups were fed regular chow and tested at 6 weeks, and 6 and 12 months of age. Glucose was measured using One Touch glucometer. Circulating levels of insulin, leptin and adiponectin were quantified by immunoassay. The results showed that leptin is higher at 6 months of age in Group 2, and is significant in females. It tends to be lower in Group 2 at 12 months, but not significant. Adiponectin on the other hand is higher at 6 weeks and 12 months in Group 2 of either sex but lower at 6 months, and concentrations at 12 months show parallel sex and group differences with the high molecular weight (HMW) form. Leptin is higher overall in males than females while adiponectin is higher in females. Results from HOMA and GTT insulin show a significant increase in both parameters at 6 weeks in males from Group 2 with a similar trend in both sexes at 6 and 12 months. We can conclude that there is an overall increase in insulin resistance in Group 2 and the group differences of leptin and adiponectin are overall inversely related, as would be expected. As part of a larger study looking at paternal transmission of epigenetic modifications to offspring, the ultimate goal is to find candidate genes for targeted intervention aimed at preventing obesity and diabetes in high‐risk offspring. Support or Funding Information Heritage College Research Award and an Ohio University Research Council Award