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Antiproliferative effect of curcumin and kaempferol on colon cancer cells
Author(s) -
Kaverimanian Arjun,
Khatwani Natasha,
Ezekiel Uthayashanker
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.841.5
Subject(s) - curcumin , phytochemical , kaempferol , colorectal cancer , chemistry , traditional medicine , cell growth , pharmacology , cancer cell , cancer , flavonoid , biochemistry , medicine , antioxidant
Results of epidemiological studies show that people who consume diets rich in phytochemicals (such as individuals living in India) have lower rates of colorectal cancer. Occasional exposure of colonic cells to a single phytochemical may be insignificant in inhibiting growth of cancer cells, whereas regular and repeated exposure of colonic cells to phytochemicals alone or in combination with other phytochemicals may be efficacious in preventing colon cancer. Combinations of phytochemicals may provide additive or synergistic effects and could be a potent way to prevent or eliminate colon cancer initiation and/or progression. The focus of this project was to determine the effect of phytochemicals alone or in combination on cancer cell proliferation. Of interest were curcumin, a phytochemical in turmeric, and kaempferol, a phytochemical in many vegetables, fruits and beans, since they both have been reported to have anti‐cancer potential. Antiproliferative effects of curcumin and kaempferol were assessed using the DLD‐1 colon cancer cell line of epithelial origin. Initial findings indicated that both phytochemicals inhibited proliferation of DLD‐1 cells to varying extents. In particular, curcumin inhibited DLD‐1 proliferation at relatively low concentrations while kaempferol showed a significant antiproliferative effect only at high concentrations. When the concentration of curcumin was kept constant at 12.5 μM (IC 50 ) and concentration of kaempferol was varied, a synergistic anti‐proliferative effect was observed. In fact, even the lowest concentrations of kaempferol combined with curcumin significantly inhibited colon cancer cell proliferation. Results of this study suggest that a combination of curcumin and kaempferol has significant inhibitory effects on proliferation of colon cancer cells. Support or Funding Information Financial support for AK, NK and TA was received from the DeNardo Education and Research Foundation.