Premium
Antioxidant activity of the hexanic extract from Eryngium carlinae “ in vitro” and in Saccharomyces cerevisiae
Author(s) -
PeñaMontes Donovan,
MejíaBarajas Jorge,
ClementeGuerrero Mónica,
SalgadoGarciglia Rafael,
CortésRojo Christian,
MontoyaPérez Rocío,
ManzoAvalos Salvador,
SaavedraMolina Alfredo
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.841.3
Subject(s) - antioxidant , chemistry , oxidative stress , dpph , lipid peroxidation , glutathione , traditional medicine , biochemistry , food science , pharmacology , biology , medicine , enzyme
Oxidative stress has been associated with the onset of chronic degenerative diseases such as cancer, cardiovascular and neurodegenerative disorders. Epidemiological studies have shown the use of plants containing antioxidant is beneficial to health. Eryngium carlinae commonly known in México as “ Hierba Del Sapo ” in traditional herbal medicine, attributed with diuretic and healing properties. Currently, ingested as daily infusion is use to regulate blood pressure and as a hypolipidemic agent; however. Different assays (“ in vitro” and “ in vivo ”) have been developed to evaluate the antioxidant activity of plant extracts. The identification and quantification of constituents, through gas chromatography–mass spectrometry (GC/MS) analysis. The antioxidant activity was evaluated using “ in vitro ” assays (total antioxidant capacity, DPPH radical scavenging activity, scavenging capacity towards hydrogen peroxide (H 2 O 2 )), and, analyzing the antioxidant effect on biomarkers of oxidative stress and its influence on the viability in cells of Saccharomyces cerevisiae treated with the hexanic extract, and exposed to H 2 O 2 . In chromatographic analysis, the majority constituents found in the hexanic extract of E. carlinae were terpenes and sesquiterpenes, (Z)β‐Farnesene (38.79 %) β‐Pinene (17.53 %), Calamene (13.30 %), α‐Farnesene (10.38 %). “ In vitro ” tests, showed a similar reducing capacity of H 2 O 2 , and an approximate effect of anti‐lipid peroxidation obtained with ascorbic acid was observed in our results. In S. cerevisiae , the results of viability are correlated in the reduction of levels of cellular lipid peroxidation, protein carbonylation and glutathione ratio in accordance with control. In conclusion, the hexanic extract of E. carlinae has antioxidant activity “ in vitro ” and in S. cerevisiae , reducing the levels of lipid peroxidation, protein carbonylation, and increasing viability, attributable to farnesene and pinene, and the synergism between the constituents of the extract. Support or Funding Information CONACYT 169093 to ASM; CIC‐UMSNH 2.16 to ASM.