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The collection and preservation of urinary proteins using a special diaper
Author(s) -
Qin Weiwei
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.825.2
Subject(s) - biomarker , urine , urinary system , medicine , economic shortage , biomarker discovery , pathology , intensive care medicine , chemistry , proteomics , biochemistry , linguistics , philosophy , government (linguistics) , gene
Change is the most fundamental property of biomarker. Homeostatic nature of internal environment limits the amount and extent of changes (biomarkers) in blood, which in turn make the urine a better biomarker source (more changes) than blood, let alone that drawing blood from infants and toddlers is hard and less tolerated. For those patients with immature or no language ability, giving chief complaint is difficult. Thus, using biomarkers in urine can provide valuable clues for diagnosis of diseases especially pediatric ones. Collecting urine from little kids and some adult patients is not as easy as from healthy adults. Here we propose a method using a special diaper to collect urine. Urinary proteins were then eluted and adsorbed onto a piece of nitrocellulose membrane which can be dried and stored in a vacuumed bag. Through this procedure, the protein patterns remain faithfully preserved in SDS‐PAGE. With this simple and economical method, it is possible to collect and preserve urine samples from infants, toddlers and special patients even on large‐scale for prospective studies which are important for biomarker research on related pathophysiological conditions. Support or Funding Information National Basic Research Program of China (973 Program)(Nos. 2012CB517605, 2013CB530805).