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Transcriptome Analysis of Conditional Knockout Mice
Author(s) -
Biefeld Chelsea,
Sztroin Kelcie,
Zhu Yi,
Yu Gang,
Dries Daniel
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.813.5
Subject(s) - conditional gene knockout , transcriptome , phenotype , knockout mouse , biology , gene , neuroscience , gene ontology , transmembrane protein , computational biology , genetics , gene expression , receptor
Gamme‐secretase is an intramembrane protein that cleaves type I transmembrane proteins. It is known to be involved in Alzheimer's disease and neurodevelopment. Previous studies have investigated the role of gamma‐secretase role in neurons however, it has seldom been considered in glial cells. Here, a conditional knockout mouse was made in which gamma‐secretase was rendered nonfunctional in oligodendrocytes. These mice displayed schizophrenic‐ and compulsive‐like phenotypes. In order to identify genes and pathways that elicit these phenotypes, RNA‐Seq was used to perform transcriptome analysis searching for differentially expressed genes in the experimental mice. Gene ontology programs were used to evaluate the genes and identify biological processes that may play a role in the behavioral aspects of these mice. Support or Funding Information We would like to thank the Howard Hughes Medical Institute, the National Institutes of Health (Grant R01 AG029547), and the International Healthspan Institute for providing funding.