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Comparison Between hPEP and spPEP Inhibition When Varying Size and Position of Inhibitory Peptide of Casein α s1
Author(s) -
Vishram Avleen Kaur,
Clack Beatrice A
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.813.4
Subject(s) - casein , peptide , pest analysis , amino acid , peptide sequence , gluten , inhibitory postsynaptic potential , biochemistry , chemistry , biology , food science , botany , gene , neuroscience
Eurygaster integriceps Puton (Sunn pest) is the most damaging insect pest of wheat in areas of North Africa, Eastern Europe and Western and Central Asia. Sunn pest injects an enzyme characterized as a prolyl endylprotease (spPEP) into wheat grains that digests the gluten protein (Darkoh et al., 2010). Previous studies have shown that casein peptides generated by Lactobacillus species have inhibitory effects on human (hPEP) and bacterial prolyl endoproteases (PEP) which makes casein a good natural candidate against spPEP. Developing biopesticides for food products from casein αs1 is complicated due to some allergenic amino acid sequences. Ruiter et al (2005) identifies a non‐allergenic peptide region on casein αs1 that includes a previously identified inhibitory sequence (LNENLLRFFVAPFPEVFG) to hPEP (Jeanneret, 2011). The Objective of this study was to produce 16 peptides derived from aS1 casein of varying lengths and varying size containing the hPEP inhibitory sequence. The inhibitory region is positioned at 0, 10, 20 and 30 amino acids from either N′ terminal or C‐terminal end. Each of the peptides were cloned into pET15b for expression with a His tag for purification. Peptides were incubated with either hPEP or spPEP in the presence of the substrate GPpNA. We report the inhibition of both enzymes based on size and placement of the inhibitory sequence. Support or Funding Information Funding: Office of Research and Sponsored Projects, SFASU and Ed and Gwen Cole, Nacogdoches, TX.