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Non‐Coding RNA Regulations in Pseudomonas aeruginosa
Author(s) -
Ammons Mary Cloud B
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.804.7
Subject(s) - quorum sensing , biofilm , pseudomonas aeruginosa , virulence , phenotype , non coding rna , bioburden , biology , gene , microbiology and biotechnology , computational biology , rna , bacteria , genetics
Bacterial biofilms are the major bioburden contributing to pathogenesis in chronic wounds such as diabetic foot ulcers, pressure ulcers, and veins leg ulcers. Mechanisms of biofilm formation are still under intense investigation and targets of precision therapeutics. Virulence and quorum sensing genes are increasingly appreciated as transcriptionally directed by non‐coding RNAs. Typically associated with eukaryotic regulation, bacterial have recently been appreciated as utilizing ncRNAs for gene and post‐transcriptional regulation. Using a RNseq and bioinformatic approach, we demonstrate significant utilization of ncRNAs in the biofilm phenotype when compared to planktonic cultures in Pseudomonas aeruginosa PAO1 biofilms. Through this analysis and network analysis, we confirm recently identified ncRNA regulation of quorum sensing and virulence factors, was well as identify novel ncRNAs associated with biofilm formation. Specifically, we find ncRNA regulation associated with the metabolic phenotype of P. aeruginosa PAO1 biofilms and confirm the transcriptional regulation of this phenotype through 1H NMR metabolomics profiling. Support or Funding Information 5K01GM103821‐04 1R03AR060995‐01 16‐746Q‐MSU‐PG51‐00, a pilot and feasibility award of parent grant 5U54GM104944‐03