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Characterizing a New Role of Novel uup gene in E.coli during DNA Repair
Author(s) -
Joung Bowon,
Chen Stefanie H,
Cox Michael M
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.792.1
Subject(s) - dna repair , mutant , biology , homologous recombination , dna damage , recbcd , replication protein a , gene , dna , microbiology and biotechnology , genetics , dna binding protein , transcription factor
Escherichia coli survival after DNA damage requires a highly coordinated suite of DNA repair mechanisms. A number of nonessential E.coli genes involved in DNA metabolism remain poorly characterized. Our recent screen identified a requirement for the uup gene in E.coli for survival after high doses of ionizing radiation. The uup gene encodes a cytosolic ATP‐binding cassette(ABC) ATPase that exhibits DNA‐binding activity in its C‐terminal domain. Null mutants of uup gene increase the frequency of transposon excision, implying more frequent replication slippage events. To further characterize the role of Uup in maintaining genomic stability, this study used a UV‐induced DNA damage assay involving other major DNA recombinational proteins, such as RecG and RecA, in combination with Uup. Under 50 kW of UV exposure, the Δ recG single mutant displayed a loss of cell viability and increased filamentation. However, the Δ recG Δuup double mutant increased the survival of Δ recG single mutant by 10 fold in rich media. In minimal media, the Δ recG Δuup double mutant exhibited an epistatic interaction for survival, relative to each Δ recG and Δuup single mutant. The Δ recA loses its capacity to repair double strand breaks via homologous recombination, leading to severe DNA damage sensitivity. Furthermore, the ΔrecA Δuup double mutant showed an additive decrease in the survival compared to Δ recA single mutant. In conclusion, this study proposes a new role of the novel uup gene in DNA replication repair in order to ensure genomic integrity.