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Coordination of Gene Expression and Cell Cycle Progression in Response to Stress; a Novel Checkpoint in S Phase
Author(s) -
Garriga Francesc Posas,
Duch Alba,
Canal Berta,
Nadal Eulàlia
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.790.1
Subject(s) - cell cycle , gene , microbiology and biotechnology , dna synthesis , dna replication , dna re replication , biology , cell cycle checkpoint , osmotic concentration , replication (statistics) , dna , genetics , control of chromosome duplication , biochemistry , virology
Exposure of cells to increases in extracellular osmolarity results in the activation of the Hog1 stress‐activated protein kinase (SAPK). Activation of Hog1 is required to generate a set of osmoadaptive responses essential for survival under high osmolarity. Adaptation to osmostress requires the induction of a large number of genes, which indicates the necessity to regulate several aspects of the cell physiology. In addition to gene expression, the SAPK also controls cell cycle. Here, we showed that the SAPK is able to modulate cell cycle delay in different phases of the cell cycle including S phase. During S phase, Hog1 controls Mrc1, a protein of the replication complex, to control DNA replication (Duch et al., 2013). The control of replication through Mrc1 is required to coordinate stress‐responsive gene induction with duplication of DNA upon osmostress. Remarkably, we have recently found that Mrc1 is also important to delay replication in response to other environmental stresses suggesting a conserved mechanism of S phase regulation upon transcriptional outbursts. Support or Funding Information This work was supported by grants from the Spanish Government (BFU2012‐33503 to F.P., BFU2014 52333 to E.d.N.) and the Fundación Marcelino Botín to F.P. F.P. and E.d.N. are recipients of an ICREA Academia (Generalitat de Catalunya).