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Maturational changes in phenotype and electrophysiological properties of Bötzinger Complex post‐inspiratory neurons of rats
Author(s) -
Moraes Davi J.A.,
Silva Melina P.,
Varanda Wamberto A.,
Machado Benedito H.
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.773.7
Subject(s) - glycine receptor , inhibitory postsynaptic potential , electrophysiology , excitatory postsynaptic potential , glutamatergic , strychnine , neuroscience , rheobase , gabaergic , biology , bursting , respiratory system , medicine , chemistry , endocrinology , receptor , glutamate receptor , glycine , anatomy , biochemistry , amino acid
Respiratory pattern is determined by chloride‐mediated conductances via GABAergic and glycinergic receptors. GABA and glycine act as inhibitory neurotransmitters in the adult central nervous system, but as excitatory neurotransmitters during postnatal development. In this study, we investigated the respiratory pattern, electrophysiological properties, phenotype and the actions of chloride‐mediated conductances on Bötzinger Complex (BötC) post‐inspiratory (post‐I) neurons on different postnatal days [neonatal, P1–5 (n=25); intermediate, P6–15 (n=39); juvenile, P15–21 (n=45); adult, P22–40 (n=57)] in in situ preparation of rats. Age‐dependent increase in both duration of expiration and post‐inspiration over the first 14 days of life were observed. Regarding the glicinergic and glutamatergic BötC post‐I neurons, burst activity, low threshold spike and calcium current increased with age. Changes in subthreshold responses were also observed, including an increase in input resistance and excitability. Rheobase current, spike frequency adaptation and action potential duration decreased progressively during postnatal development. The transition from excitatory to inhibitory effects of GABA and glycine on BötC post‐I neurons occurred at approximately P6. The age‐dependent changes in the actions of chloride‐mediated conductances are mediated by the development of chloride cotransporters (KCC2, NKCC1 and NKCC2) in BötC post‐I neurons. We conclude that postnatal maturation of the electrophysiological properties of BötC post‐I neurons may explain the development changes in the respiratory pattern and respiratory control of upper airway resistance in rats. Support or Funding Information FAPESP