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Caffeine improves survival in autoresuscitation‐impaired serotonin deficient rats
Author(s) -
Lee Stella,
Nattie Eugene
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.773.5
Subject(s) - caffeine , bradycardia , apnea , nicotine , medicine , anesthesia , heart rate , peripheral chemoreceptors , hypoxia (environmental) , stimulant , chemoreceptor , chemistry , receptor , organic chemistry , oxygen , blood pressure
Our previous investigation demonstrated that an existing underlying vulnerability in rat neonates (i.e. brainstem 5‐HT deficiency) in combination with a stressor (i.e. pre‐ and early post‐natal nicotine exposure) exacerbates autoresuscitation failure from an anoxic event at a critical developmental period (P10 rats). During this critical period, we hypothesize that caffeine, a respiratory stimulant, will improve successful autoresuscitation rates in the 5‐HT deficient, nicotine exposed P10 neonates and reduce mortality. Preliminary data demonstrate that an intraperitoneal injection of 10mg kg −1 caffeine prior to anoxic challenge improves autoresuscitation rates (survival rate approximately 40%) in the 5‐HT deficient, nicotine exposed P10 rats in comparison to the rats who received IP saline vehicle injections (survival rate of 25%). Prior investigation suggests that eupnea and heart rate recovery following an anoxic event (which elicits an apnea accompanied by a bradycardia) is significantly delayed in 5‐HT deficient rats treated with nicotine, making them more susceptible to failure of autoresuscitation (p<0.05). Presence of caffeine is likely facilitating successful autoresuscitation by shortening apnea duration and promoting an early onset of gasping post‐apnea for a faster eupnea and heart rate recovery. Support or Funding Information Research is supported by the NIH Program Project Grant HD036379 (NICHD, PI, Dymecki, S. M.; Project PI, Nattie, E. E.). The authors would also like to acknowledge the generous support of the Tenney Fund and the Ryan Fellowship (awarded to S. Lee).