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Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) is Crucial for CO 2 Signal Transduction in Avian Intrapulmonary Chemoreceptors (IPC)
Author(s) -
Molloy Rhett Gordon,
Low Trevor Michael,
Bassett Peter Ross,
Lonjaret JeanGuillaume,
Hempleman Steven Curtis
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.772.4
Subject(s) - cystic fibrosis transmembrane conductance regulator , glibenclamide , chemistry , signal transduction , transduction (biophysics) , transmembrane protein , ion channel , ion transporter , biophysics , microbiology and biotechnology , pharmacology , medicine , endocrinology , biochemistry , biology , receptor , membrane , gene , diabetes mellitus
CFTR is an anion channel and ABC transmembrane protein which also modulates other anion channels and transporters. Since CFTR is widely expressed in cells that transport anions, and we hypothesize that anion transport in IPC is critical for CO 2 transduction, we tested for the presence of CFTR in IPC. Glibenclamide, a non‐selective CFTR inhibitor, and CFTR‐172inh, a highly specific CFTR inhibitor were given separately to pentobarbital‐anesthetized mallards ( Anas platyrhynchos , n =15) while recording single cell IPC spike train responses to inspired CO 2 steps (0%–7%, 11sec period). Both drugs produced similar results, where IPC discharge with the lowest 2 μmol/kg doses was unchanged from control, while the 4 and 8 μmol/kg doses significantly decreased both mean IPC discharge and phasic adaptation (ANOVA, p<0.01). IPC CO 2 sensitivity was completely abolished in 4 CFTR‐172inh experiments and 4 glibenclamide experiments. Results suggest that CFTR is a crucial anion regulating channel involved in IPC CO 2 signal transduction. Support or Funding Information Support: NIH R15 HL087269‐02.