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Extracellular matrix affects endothelial cell attachment under shear flow
Author(s) -
Chiu YiFan,
Huang Bin
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.763.26
Subject(s) - fibronectin , shear (geology) , chemistry , extracellular matrix , biophysics , adhesion , sodium hydroxide , coating , microbiology and biotechnology , materials science , biomedical engineering , composite material , biochemistry , biology , medicine , organic chemistry
Shear flow plays an important role in cardioprotection. In the present In vitro study, shear flow is processed on a glass slide that coated with biomatrix so that the endothelial cells (ECs) can grow on it and then exposed to the shear flow. However, the detachment of ECs always hinders scientists to study the molecular aspect of cardioprotective mechanism. In the present study, the glass slides were washed either by hydrochloride (acidic wash) or sodium hydroxide (basic wash). After sterilized by ethanol, the glass slides were coated with three matrixes, poly‐L‐Lysine, fibronectin and collagen separately. Exposed the ECs to the shear flow of 5 dynes/cm 2 and 20 dynes/cm 2 for 2 hours, we investigated the attached cell number and their viability. The results showed that basic wash coupled with the coating of fibronectin has the best binding efficiency as compared to the others. The sustained expression of focal adhesion kinase (FAK) also supports our observation. As a result, our study provides a considerable application for the research of shear flow. Support or Funding Information MOST 104‐2632‐M‐037‐001, KMU‐TP104E13, KMU‐O104003, KMU‐O105001