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Molecular phenotypes of gills from resident marine versus landlocked freshwater three‐spined sticklebacks ( Gasterosteus aculeatus )
Author(s) -
Li Johnathon,
Paguio Darlene May,
Kültz Dietmar
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.760.21
Subject(s) - gasterosteus , gill , biology , population , zoology , stickleback , proteome , three spined stickleback , ecology , fishery , fish <actinopterygii> , bioinformatics , demography , sociology
To analyze the influence of marine versus freshwater (FW) habitats on branchial proteomes of three‐spined sticklebacks, we compared a resident marine (Bodega Harbor, CA) with a landlocked FW (Lake Solano, CA) population. The marine population represents a classical fully plated morphotype whereas the FW population represents a typical low‐plated morphotype as assessed after staining fish with Alizarin Red and analysis of morphometric parameters that define stickleback morphotypes. Unbiased label‐free proteomics was used to identify and quantify over 1700 branchial proteins. Thirty four proteins identified to differ significantly (p<0.05) and by more than 2‐fold in abundance were targeted for data‐independent acquisition and quantitative tandem mass spectrometry (MS2) reaction monitoring with Skyline. Proteins confirmed to be significantly different in abundance were then further analyzed for gene ontology enrichment to assess population‐specific gill functions. Ion transport, oxidative metabolism and redox regulation were significantly enriched in gills of marine sticklebacks while cell/epithelial morphogenesis and proteolysis were significantly enriched in FW sticklebacks. We conclude that these branchial functions and the proteins that mechanistically support them are strong targets of selection when marine sticklebacks invade FW habitats. Support or Funding Information National Science Foundation IOS‐1355098.