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Species Differences in Gastrointestinal Tract Glucose Transporters between Rainbow Trout ( Oncorhynchus mykiss ) and Nile Tilapia ( Oreochromis niloticus )
Author(s) -
Subramaniam Marina,
Weber Lynn P,
Ching John C,
Enns Cole B,
Kilgour Alyssa B,
Drew Murray D,
Loewen Matthew E
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.760.20
Subject(s) - rainbow trout , oreochromis , nile tilapia , biology , midgut , trout , tilapia , transporter , glucose transporter , biochemistry , fishery , endocrinology , ecology , fish <actinopterygii> , larva , gene , insulin
Metabolic differences in omnivore and carnivore glucose homeostasis are well established. However, differences in the gastrointestinal glucose transporers have not been well characterized. Here we assess the differences in electrogenic transport of D‐glucose along different sections of the intestine between Rainbow trout ( Oncorhynchus mykiss ), a carnivorous fish and Nile tilapia ( Oreochromis niloticus ), an omnivorous fish in Ussing chambers. The kinetics of glucose transport in pyloric caeca and midgut of trout were similar to the sodium‐dependent glucose transporter isoform 1 (SGLT1) in mammals, and the kinetics in colon resembled mammalian SGLT2. In contrast to trout, the kinetics in all three intestinal sections in tilapia resembled mammalian SGLT2. In addition, SGLT1 and SGLT2 inhibitors further confirm the locations of glucose transporters. In conclusion, trout has two types of SGLTs, a high affinity, low capacity transporter in pyloric caeca and midgut, and a low affinity, high capacity transporter in the colon. Tilapia has only one type of SGLT throughout the gut, which is a low affinity, high capacity transporter Support or Funding Information NSERC ‐ CRDPJ: 446912‐13 to LPW, MEL & MDD