Premium
Pulsed Ultrasound Stimulation Prevents Bacterial Lipopolysaccharide Induced Muscle Wasting and p38 MAPK Phosphorylation in Mouse C2C12 Skeletal Myotubes
Author(s) -
Ueno Mizuki,
Maeshige Noriaki,
Hirayama Yusuke,
Nakanishi Ryosuke,
Yoshikawa Madoka,
Fujino Hidemi
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.745.3
Subject(s) - myogenesis , skeletal muscle , stimulation , myofibril , p38 mitogen activated protein kinases , muscle atrophy , medicine , myocyte , lipopolysaccharide , endocrinology , c2c12 , phosphorylation , cachexia , mapk/erk pathway , chemistry , microbiology and biotechnology , biology , cancer
Cachexia is a multifactorial syndrome characterized by involuntary weight loss due to skeletal muscle wasting and fat depletion. In cachectic state, inflammatory cytokines increase systemically, and following p38 MAPK phosphorylation leads to muscle protein degradation. While pulsed ultrasound stimulation can provide the mechanical stimulation to the target tissue, and has been reported to show anti‐inflammatory effects. Therefore, the preventive effect of pulsed ultrasound stimulation on muscle protein degradation induced by lipopolysaccharide (LPS) using C2C12 was investigated in this study. Pulsed ultrasound stimulation was performed immediately before LPS treatment. The pulsed ultrasound signal consisted of a series of 3 MHz, 20% burst rate, and was delivered at an intensity of 0.5 W/cm 2 for 30 sec. LPS treatment resulted in the increase of p38 MAPK phosphorylation and myofibril protein degradation. In addition, the treatment induced the decrease of cell viability and diameter in C2C12 myotubes. Meanwhile, pulsed ultrasound stimulation prevented myofibril protein degradation induced by LPS in C2C12 myotubes, and inhibited the phosphorylation of p38 MAPK. These results indicate that mechanical stimuli with pulsed ultrasound could be a preventive intervention against cachectic muscle atrophy.