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Investigation of MicroRNA Expression Profiles in the Susceptible Population to In‐stent Restenosis
Author(s) -
Chen ChangJui,
Wu ChiehHsi,
ChunMing Shih,
Huang ChunYao,
Bi WeiFung,
Chan ChaoShun,
Kao YungTa,
Pan ChunHsu,
Chiang ShuoJu,
Chiang KuangHsing,
Liu YunRu,
Luo JiDung
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.743.3
Subject(s) - restenosis , microrna , medicine , population , stent , pathological , angioplasty , oncology , bioinformatics , biology , genetics , gene , environmental health
In‐stent restenosis (ISR) is a pathological phenomenon in response to vascular injury after balloon angioplasty with stent placement, which occurs in just about 20~30% cases. Because of the lack of ISR‐specific clinical biomarkers, the drug‐eluted stents (DES) were widely abused as preventive interventions at present. A growing number of evidences suggested that abnormal expression profiles of microRNAs (miRNAs) associated with development of numerous diseases. To date, no reports showed that any miRNA candidates have been used to distinguish the population with high‐risk for ISR. Thereby, the purpose of the present study was to identify potential candidates of miRNAs differentially expressed in ISR patients. Based on the correlation network of our preliminary data from the proteiomics analysis of animal disease model and a small‐size SNP array of human samples, the candidate miRNAs with highly potential to involve in ISR development were picked up by using the MetaCore™ software. Thirty participants, including the restenosis group and the non‐restenosis group, were recruited from the Taipei Medical University Hospital, Taiwan. Total miRNA was extracted from blood serum of each participant, and expression profiles of five candidate miRNAs (mir‐142‐5p, mir‐132‐3p, mir‐24‐2‐5p, mir‐424‐5p and mir‐15b‐5p) were analyzed by using SYBR Green‐based real‐time PCR. Our data mentioned that the expression levels of two mRNAs (mir‐424‐5p and mir‐15b‐5p) showed increasing trends but another mRNAs (mir‐142‐5p, mir‐132‐3p and mir‐24‐2‐5p) were decreased in the restenosis group. Support or Funding Information Taipei Medical University Taipei Medical University Hospital