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A Newly Developed Technique of in vivo Synchrotron Radiation Renal Micro‐angiography in Spontaneously Hypertensive and Normotensive Rats
Author(s) -
Miya Ken,
Matsushita Shonosuke,
Hyodo Kazuyuki,
Tokunaga Chiho,
Sakamoto Hiroaki,
Mizutani Taro,
Hiramatsu Yuji
Publication year - 2016
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.30.1_supplement.739.3
Subject(s) - in vivo , kidney , angiography , medicine , pathology , nuclear medicine , biology , microbiology and biotechnology
Although real‐time imaging of renal arterioles and glomeruli is desirable, conventional angiography is not capable of such visualization, due to limitations in spatial resolution. We have developed a novel method of visualizing renal arterioles and glomeruli in real‐time in in‐vivo rats, without exteriorizing kidneys, utilizing synchrotron radiation (SR) derived X‐rays. In this study, we aimed to detect morphological differences in the kidney by utilizing SR renal micro‐angiography (SRRM) in normotensive and hypertensive rats. Method SRRM was performed at the Photon Factory of the High Energy Accelerator Research Organization (Tsukuba, Japan). The SR derived X‐rays are characterized by high collimation and photon flux, thus resulting in higher spatial resolution compared with conventional X‐ray. Male Wistar rats (WR; n=5) and spontaneously hypertensive rats (SHR; n=5) were anesthetized and iodine contrast agent was administrated via polyethylene tube for angiography inserted into the infra‐renal aorta via laparotomy with its tip just above renal arteries. Result In both groups, renal arterioles and glomeruli were clearly visualized ( Fig. 1). The average diameter of glomeruli in the WR group was 197±42 micrometer and 193±37 micrometer in the SHR group, respectively. It was also possible to detect local stenosis of interlobular arterioles in SHR rats with diameters of 50 micrometer. Compared with the WR group ( Fig. 2), perfusion of contrast agent was apparently decreased in the SHR group ( Fig. 3). Conclusion The result shows the effectiveness of SRRM for visualizing renal arterioles and glomeruli in in‐vivo rats, in real‐time, without exteriorizing kidneys. The SRRM is a promising method for the investigation of renal physiology as well as pathology.